Tomasz Ciach PhD Eng. Professor
 
Beata Butruk-Raszeja PhD Eng.
 
Magdalena Janczewska MSc Eng.
 
Katarzyna Każmierska MSc Eng.
Kamil Kopeć MSc Eng.
 
Piotr Kowalczyk MSc Eng.
 
Martyna Kucharska PhD Eng.
 
Aleksandra Kuźmińska, MSc Eng.
 
Aleksandra Kulikowska MSc Eng.
 
Ilona Łojszczyk MSc Eng.
 
Aleksandra Mościcka-Studzińska MSc Eng.
 
Rafał Podgórski MSc Eng.
 
Aleksandra Poniatowska MSc Eng.
 
Agata Stefanek MSc Eng.
 
Paulina Trzaskowska MSc Eng.
 
Maciej Trzaskowski MSc Eng.
 

Iga Wasiak MSc Eng.

 
Michał Wojasiński MSc Eng.
 

 

Polysaccharide nanoparticles

Recently, nanotechnology is rapid growing science field. In this particular field of knowledge, we are focused on synthesis of nanoparticles and nanofibers by electrohydrodynamic atomization method and by chemical synthesis “bottom-up”.

In our laboratory the technology of synthesis of polysaccharide nanoparticles for targeted drug delivery to tumors was developed. Our synthesis is based on specific oxidation of polysaccharide chain and adding to the polysaccharide active groups of hydrophobic molecules.

Nanoparticles are synthesized in water solutions based on hydrophobic effect of side groups with hydrophilic chains of polysaccharides in water environment. This leads to the formation of nanoparticles by self-assembly mechanism. Nanoparticles obtained in this way might be modified with drugs or antibodies.

Polysaccharide shell on nanoparticles protects nanoparticles from the immune system detection and adhesion of small peptides from the plasma.

Due to the fact, that cancer cells have altered mitochondrial metabolism, they use low efficient glucose anaerobic metabolism (Warburg effect). This effect combined with high growth ratio generate high glucose demand in cancer cells, which is about 200 times higher than in basic tissue cells. The Warburg effect is used in tumor diagnostics with PET technique, when the patient receives 2-deoxyglucose marked with fluorine isotope. Designed in BioMedLab nanoparticles use the same effect because of polysaccharide outer shell, recognized by glucose transporters in cancer cells. This leads to accumulation of nanoparticles only in tumors, not in cells of healthy tissues. Test performed on mice typically results with about 5 times higher drug concentration in tumor rather than other tissues.

Accumulation of Doxorubicin provided in nanoparticles comparing Doxorubicin in the same dose provided typically. This test is based on self fluorescence of the drug.

Due to accumulation of nanoparticles carrying drug in tumor, cancer treatment might have potentially no side effects, comparing to distribution of drug in pure form, which results with very strong side effects. However, confirmation of this hypothesis requires further research.

Comparison of side effects (by mass of organs) in case of Doxorubicin and the same dose provided in nanoparticles.

Comparison of side effects (by body mass of mouse) in case of Doxorubicin and the same dose provided in nanoparticles.

Application of polysaccharides nanoparticles designed in BioMedical Engineering Laboratory in Faculty of Chemical and Process Engineering of Warsaw University of Technology gives hope for improvement in treatment with currently known and used cytotoxic drugs and reduction of siide effects of the teraphy. However, until we fully develop this system, there is still a few years of intensive research ahead of us.

 
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